ENDONUCLEASE BAMFI Meaning and
Definition
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Endonuclease BamHI is a specific type II restriction enzyme that is commonly found in the bacterium Bacillus amyloliquefaciens. It is widely used in molecular biology research and genetic engineering applications. This enzyme recognizes a specific DNA sequence, known as the recognition site, and cleaves the DNA molecule at that site. The recognition site for BamHI is characterized by the sequence 5'-GGATCC-3'. The enzyme cuts the DNA precisely between the second and third nucleotides in this sequence, resulting in a staggered double-stranded DNA break with overhanging ends referred to as "sticky ends". These sticky ends are useful in DNA manipulation techniques such as cloning, as they allow for the easy insertion of a DNA fragment into another DNA molecule with a complementary sticky end.
Endonuclease BamHI belongs to the class of type II restriction enzymes, which are characterized by their ability to recognize specific DNA sequences and cleave at defined positions within these sequences. This enzyme is commonly used in molecular biology laboratories due to its high specificity and efficiency for DNA cleavage. In addition, it can be easily obtained from commercial sources or through recombinant DNA technology.
Endonuclease BamHI plays a crucial role in numerous molecular biology techniques, including restriction enzyme digestions, DNA sequence analysis, gene cloning, and recombinant DNA technology. Its versatility and ease of use have made it an invaluable tool in genetic engineering, biomedical research, and various other scientific fields that employ DNA analysis and manipulation.
Common Misspellings for ENDONUCLEASE BAMFI
- wndonuclease bamfi
- sndonuclease bamfi
- dndonuclease bamfi
- rndonuclease bamfi
- 4ndonuclease bamfi
- 3ndonuclease bamfi
- ebdonuclease bamfi
- emdonuclease bamfi
- ejdonuclease bamfi
- ehdonuclease bamfi
- ensonuclease bamfi
- enxonuclease bamfi
- enconuclease bamfi
- enfonuclease bamfi
- enronuclease bamfi
- eneonuclease bamfi
- endinuclease bamfi
- endknuclease bamfi
- endlnuclease bamfi
- endpnuclease bamfi
Etymology of ENDONUCLEASE BAMFI
The word "endonuclease BamFI" is derived from the names of the bacteria from which the enzyme is isolated, as well as the type of enzyme it is.
1. "Endonuclease": The term "endonuclease" comes from the Greek words "endon" (meaning "within") and "nucleus" (meaning "kernel" or "seed"). It refers to an enzyme that cleaves the phosphodiester bonds within nucleic acids, such as DNA or RNA, at specific sites.
2. "BamFI": The term "BamFI" is a specific designation given to the endonuclease enzyme. The name "Bam" is derived from the names of two researchers, Richard J. Roberts and Jonathen M. Morrow, who first discovered and characterized this particular enzyme.
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